Network Pharmacology Analysis of the Intervention Effects of Ganoderma lucidum Mycelium Powder Ethanol Extract on Non-Alcoholic Fatty Liver Disease

LIN Juan-juan; JIANG Yong-mei

doi:10.13651/j.cnki.fjnykj.2025.10.004

Abstract：The intervention mechanism of ethanol extract(DA)of Ganoderma lucidum mycelium powder on the non-alcoholic fatty liver disease(NAFLD)was systematically explored by using the network pharmacology method in this study.The components of DA were identified by using LC-MS,and its active ingredients were screened based on ADME models.The targets of these active ingredients were predicted via the Swiss Target Prediction platform,while NAFLD-related targets were retrieved from the Gene Cards and OMIM databases.The PPI network was constructed by using the Cytoscape software to screen the core targets,and then the enrichment analysis was carried out based on the DAVID database.Finally,AutoDock was used to complete the molecular docking validation.The results of this study indicated that 115 compound components were identified from DA by LC-MS,and 53 active ingredients were screened by Swiss platform.These active ingredients and NAFLD exhibited 250 shared targets,from which 26 core targets were identified through screening via the Protein-Protein Interaction(PPI)network.The enrichment analysis revealed that these targets were significantly enriched in the signaling pathways such as PI3K-Akt,MAPK,and AGE-RAGE,and were involved in the processes including inflammatory response,signal transduction,and transcriptional regulation.The molecular docking demonstrated that the core components(such as DA39)exhibited good binding affinity with the targets.This study has shown that DA played an anti-NAFLD effect in improving insulin resistance,regulating lipid metabolism,reducing inflammation,and countering fibrosis through the synergistic effect of"multi-compound-multi-target-multi-pathway",and reflecting the holistic therapeutic characteristics of traditional Chinese medicine.