Cloning and Bioinformatics Analysis of CRY1 Gene in Casuarina equisetifolia
LI Long-yu
GAO Ping-ru
ZHENG Wen-hui
HE Wen-jin
CHEN Jian-nan
Abstract：Cryptochrome is an important blue light receptor affecting the plant growth.In order to explore the mechanism of cryptochrome protein of Casuarina equisetifolia in the light regulation network,the CRY1 gene sequence of Casuarina equisetifolia was screened and identified from the Casuarina equisetifolia genome database and named CeCRY1.The full-length cDNA sequence of CeCRY1 gene from Casuarina equisetifolia was cloned by PCR experiment,and the CeCRY1 protein was transiently expressed in tobacco cells by agrobacterium-mediated transformation method,thus to carry out the subcellular localization analysis,and then the bioinformatics analysis of CeCRY1 protein was performed.The results showed that the full-length cDNA sequence of CeCRY1 gene from Casuarina equisetifolia was 2 046 bp,and the number of amino acids encoded by CeCRY1 gene was 681.The molecular weight of the protein was 76.60 kDa,and the isoelectric point PI was 5.64.The subcellular localization analysis showed that CeCRY1 protein was localized in the nucleus and cytoplasm of tobacco cells.The phylogenetic tree analysis showed that CeCRY1 protein was closely related to CRY1 proteins in some species of Salicaceae and Morus notabilis of Moraceae.
Keywords：CryptochromeCasuarina equisetifoliaCloningBioinformatics analysis
Publication Date：2025-01-27
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 48-55 )