Mechanism of Lactobacillin PlnJ(G5)on Alleviating the Intestinal Epithelial Cells and Intestinal Inflammation in Mice Induced by LPS
XU Li-hui
YAN Meng-han
WANG Quan-xi
Abstract：In order to explore the mechanism of lactobacillus PlnJ(G5)modified and preserved in the laboratory on the intestinal epithelial cells IPEC-J2 and the intestinal inflammation in mice induced by lipopolysaccharide LPS,the intestinal epithelial cell IPEC-J2 and model animal mice were selected as the research carriers.LPS was used to establish an inflammatory model,and PlnJ(G5)was added for the treatment.Then,the cell viability was detected by cck-8 and the expression changes of inflammatory cytokines was detected by the real-time fluorescence quantitative PCR.The results showed that 5.0 μg·mL-1 LPS had little effect on the cell viability of IPEC-J2,and the mRNA expression levels of IL-1,IL-6,TNF-α,IFN-α and IFN-β in IPEC-J2 cells treated with this concentration were significantly higher than those in the blank control group,which proved that the inflammatory model of IPEC-J2 cells induced by LPS was successful.The results of CCK-8 detection showed that the safe additive dosage of PlnJ(G5)for IPEC-J2 cells was 20.0 μg·mL-1.The results of qPCR detection showed that after IPEC-J2 cells treated with 5.0 μg·mL-1 LPS for 4 h,the addition of 20.0 μg·mL-1 unheated PlnJ(G5)had the best inhibitory effect on the mRNA expression of IL-1,IL-6,TNF-α,IFN-α and IFN-β.The experimental results showed that PlnJ(G5),which was not heat treated,had the best anti-inflammatory effect on mice induced by LPS.
Keywords：LactobacillinLipopolysaccharideInduceIntestinal epithelial cellsInflammation
Publication Date：2024-10-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 36-42 )