Screening and Identification of a Wild Cellulose-degrading Strain with High Efficiency and Determination of Its Enzyme Activity
LIAO Kai
XIAO Tian
YAN Jian-hong
ZHENG Hui-xin
JING Yuan-rong
LIU Xiu
NI Xian-zhi
JIN Chen-zhong
GUO Kai-fa
Abstract：In order to isolate cellulase-producing bacteria from the overrotten soil and further study the cellulose degradation ability of the strain,a wild cellulose-degrading strain ZG2-3 was screened by using the carboxymethyl cellulose (CMC)-congo red staining method.Then,the 16 S rRNA molecular identification was used to identify the species,and four commercial cellulose-degrading bacteria strains were screened from the promoting-decomposing agent.Last,the enzyme activity differences between the wild cellulose-degrading bacteria and the commercial cellulose-degrading bacteria were compared.The results showed that one strain of bacteria with high cellulose degradation ability,ZG2-3,was screened by using the carboxymethyl cellulose (CMC)-congo red staining method,which was collected and isolated from the overrotten soil under the osmanthus forest in Zhushan Park,Loudi City.ZG2-3 was identified as Bacillus subtilis.The carboxymethyl cellulase (CMCase) activity and filter paper enzyme (FPA) activity of ZG2-3 were 0.054 IU·mL-1 and 0.02 IU·mL-1,respectively,and the CMCase activity of ZG2-3 was significantly higher than that of the commercial cellulose-degrading bacteria.
Keywords：CelluloseBacillus subtilisScreeningIdentificationEnzyme activity
Publication Date：2024-05-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 16-19 )