Technology Optimization for the Production of Glutathione Fermented by Saccharomyces cerevisiae
LIN Wei-ling
ZHU Hong-yang
LI Yong-ning
Abstract：In order to explore the optimal technological conditions for the production of glutathione(GSH)fermented by Saccharomyces cerevisiae,based on the single factor experiment,the liquid fermentation medium was optimized through Plackett-Burman design experiment combined with the response surface methodology.The results showed that the optimal culture medium for the production of GSH fermented by Saccharomyces cerevisiae was as follows:glucose of 71 g·L-1,yeast extract paste of 4 g·L-1,NH4Cl of 6 g·L-1,KH2PO4 of 2.0 g·L-1,MgSO4 of 0.5 g·L-1,pH of 6.0.After three batches of parallel experiments,the mean value for the actual yield of GSH was 80.5 mg·L-1,which was similar to the predicted value and increased by about 19.2％higher than that before optimiza-tion(65.03 mg·L-1).
Keywords：Saccharomyces cerevisiaeGlutathionePlackett-Burman designResponse Surface analysisOptimization
Publication Date：2023-12-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 27-34 )