Interaction and Bioinformatics Analysis of CRY1 and COP1 in Isochrysis Galbana
LI Huan
LI Long-yu
ZHONG Jie
CHE You-qiang
YU Xue-bing
HE Wen-jin
CHEN Jian-nan
Abstract：In order to verify whether the cryptochrome protein IgCRY1 of Isochrysis galbana interacted with the downstream protein IgCOP1,the total RNA of Isochrysis galbana was extracted and reverse transcribed into cDNA as a template to amplify the target fragment.The BD-IgCRY1 and AD-IgCOP1 vectors were constructed and co-trans-fected into the yeast strain AH109 for the yeast two-hybrid verification.And the bioinformatics analysis of IgCOP1 protein was performed.The results showed that the CDS sequences of IgCRY1 and IgCOP1 cloned from Isochrysis galbana were 1 560 bp and 1 185 bp,respectively.The mutual interaction between IgCRY1 protein and IgCOP1 pro-tein was verified by the yeast two-hybrid system.The bioinformatics analysis of the interacting protein IgCOP1 re-vealed that the molecular size of the protein was 43.7 kDa,and the number of encoded amino acids was 394,which was a typical hydrophilic protein with 62.69％of its secondary structure irregularly coiled.The phylogenetic tree a-nalysis showed that IgCOP1 protein was clustered at the bottom of the branch,and had a distant relationship with the other branches.The verification of the interactions between IgCRY1 protein and IgCOP1 protein provided an impor-tant theoretical basis for the biological process of light signal regulation in Isochrysis galbana.
Keywords：Isochrysis galbanaYeast two-hybridIgCRY1 proteinIgCOP1 proteinBioinformatics analysis
Publication Date：2023-12-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 7-14 )