Creation of Novel Glutinous Rice Germplasm of Fuxiangzhan(FXZ)Using Genome Editing
WEI Lin-yan
FAN Jia-xing
ZHANG Min
WANG Jin-lan
XIE Hong-guang
WEI Yi-dong
ZHANG Jian-fu
Abstract：Gene editing is an emerging genetic improvement technology that allows for rapid and precise modification of crop traits.FXZ is a newly developed high-quality,blast-resistant,long-grain indica rice variety with great potential for commercialization.This study aimed to utilize gene editing technology to create FXZ glutinous rice materials rapid-ly.By screening and optimizing target sequences,the rice granule-bound starch synthase gene(Waxy,Wx)was suc-cessfully knocked out using CRISPR/Cas9 technology,resulting in Wx gene knockout mutants.Through progeny ag-ronomic traits evaluation and amylose content determination,the results showed that the amylose content of the knockout materials decreased from 16.3％to approximately 1.67％-1.81％,meeting the criteriaof glutinous rice.Additionally,there were no significant changes observed in plant morphology,yield,and resistance in the mutant plants.This study provides a new approach for the application and promotion of FXZ and enriches the genetic re-sources of blast-resistant glutinous rice.
Keywords：Gene editingGlutinous riceFuxiangzhan(FXZ)Granule-bound starch gynthase gene
Publication Date：2023-10-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 34-39 )