Screening and Identification of Lipase-producing Strains
CHEN Mei-lian
BAI Rui-xuan
LIN Lin
Abstract：In order to explore more lipase-producing strain resources,324 fungal strains were isolated and screened from the plants in seawater,coastal wetland & mud soil and coastal areas by high throughput through the olive oil plate detection(transparent circle observation).Forty-eight candidate lipase-producing strains were screened on the plate with pH 9.4.Through the ITS sequence analysis,12 strains were preliminarily identified as Fusarium,Peni-cillium,Cladosporium,Alternaria,etc.,most of which were Cladosporium and Penicillium.The fermentation broth of the two strains(# 89 and # 260)cultured for 24 h was further detected by using the titration method of so-dium hydroxide.The lipase activity produced by #89 strain was(6.92±2.40)U·mL-1,and the lipase activity produced by # 260 strain was(7.67±1.70)U·mL 1.
Keywords：LipaseStrain resourcesITS sequenceScreening
Publication Date：2023-09-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 63-68 )