Application of KASP Technology in Animal Resource Identification and Marker-assisted Breeding
FU Hong-gao
YANG Li-cheng
WU Rui-qiong
HUANG Zhen
Abstract：Kompetitive allele-specific PCR(KASP)was a fluorescence-based homogeneous genotyping technique,which could perform the biallelic genotyping detection on the single nucleotide polymorphism sites and insertion/dele-tion polymorphisms in the genome.It had the advantages of rapid detection,low cost and high accuracy.The compo-sition,amplification principle and reaction steps of KASP detection system were described in this paper,as well as its advantages over the traditional SNP detection methods.Then,the current research progresses in animal genetic iden-tification,functional gene analysis and molecular marker-assisted selection(MAS)assisted breeding were summa-rized,in order to provide reference for the application of KASP technology in animal field.
Keywords：KASPSNPGenetic identificationMolecular marker-assisted breeding
Publication Date：2023-09-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：10( 32-41 )