Antitumor component-Ⅰ in Agkistrodon halys venom inhibits proliferation and migration of cisplatin-resistant gastric cancer cells by downregulating RAI14
LI Yanyu
LI Chen
DAI Chuanjun
GUO Runzhi
HAN Haoyu
LU Linming
ZHOU Fangfang
ZHI Hui
Abstract：Objective To evaluate the inhibitory effect of antitumor component-Ⅰ in Agkistrodon halys venom(AHVAC-I)on proliferation and migration of cisplatin-resistant gastric cancer cells and explore the underlying mechanism.Methods Cisplatin-resistant MKN-28(MKN-28/DDP)cells were obtained by continuous exposure of MKN-28 cells to stepwise-increasing concentrations of cisplatin.MKN-28/DDP cells were treated with different concentrations of AHVAC-I,and the changes in proliferation,migration and invasion of the cells were examined with colony-forming assay,CCK-8 assay wound-healing assay,and Transwell assay.Western blotting was performed to examine the effect of AHVAC-I on expressions of epithelial-mesenchymal transition(EMT)markers of MKN-28/DDP cells;the changes in protein and mRNA expression of retinoic acid induced 14(RAI14)was detected with Western blotting and qRT-PCR.Results Treatment with 2,4,and 8 μg/mL AHVAC-I significantly inhibited proliferative,migratory and invasion abilities and reduced the expressions of EMT markers in MKN-28/DDP cells.Compared with MKN-28 cells,MKN-28/DP cells showed an increased expression of RAI14,which was significantly lowered after treatment with AHVAC-I.Supplementation with exogenous RAI14 obviously attenuated the inhibitory effect of AHVAC-I on proliferation and migration of MKN-28/DDP cells.Conclusion AHVAC-I decreases proliferation and invasion of MKN-28/DDP cells by downregulating RAI14 expression.
Keywords：Agkistrodon halys venomgastric cancercisplatin resistanceinvasionproliferationretinoic acid induced 14
Publication Date：2026-01-20
Online Publishing Date：2026-01-22(First online date of this platform, not the publication date of the document)
Pages：9( 113-121 )