High-throughput circular RNA sequencing reveals tumor-specific high expression of hsa_circ_0001900 in Wilms tumor in association with poor prognosis
GAO Zhiqiang
LIN Jie
HONG Peng
HU Zaihong
CUI Kongkong
WANG Yu
DONG Junjun
SHI Qinlin
TIAN Xiaomao
WEI Guanghui
Abstract：Objective To explore the expression profile of circular RNAs(circRNAs)and their potential roles in prognosis and progression of Wilms'tumor(WT).Methods Four pairs of WT and adjacent tissues were collected for high-throughput circRNA sequencing to identify the differentially expressed circular RNAs.RT-qPCR was used to verify the expression levels of the top 6 candidate circRNAs in the clinical samples.hsa_circ_0001900 was selected for analysis of its correlation with clinicopathological features and prognosis in 34 patients with WT.Sanger sequencing and RNase R digestion experiments were used to verify the cycling site and structural stability of hsa_circ_0001900 molecule.Results A total of 23 978 circular RNA molecules were identified in WT tissues by high-throughput circular RNA sequencing,and among them 614 were differentially expressed in WT.hsa_circ_0001900 showed the highest expression level among the differentially expressed circRNAs,which was consistent with the findings in clinical tumor samples and the sequencing results.Correlation analysis showed that hsa_circ_0001900 expression level was positively correlated with WT volume,and the children with high hsa_circ_0001900 expression had a lowered recurrence-free survival rate.The results of Sanger sequencing verified the circular splice site sequence of the molecule,and Rnase R digestion assay confirmed its stable covalent structure.Conclusion This study presents a comprehensive expression profile of circular RNAs in WT,and the expression level of hsa_circ_0001900 is related to the size of WT and the patients'prognosis,suggesting its possible role as a key driving gene in WT progression.
Keywords：nephroblastomaWilms tumorcircular RNAhsa_circ_0001900
Publication Date：2025-11-20
Online Publishing Date：2025-12-05(First online date of this platform, not the publication date of the document)
Pages：9( 2466-2474 )