Polydopamine-modified phycocyanin nanoparticles with photothermal antimicrobial activity promote skin wound healing in mice

ZHANG Chen; XU Zhi; LI Xiang; HE Pengyixiang; QU Kailin; NING Qi; JIN Yile; YANG Surui; WU Xu

doi:10.12122/j.issn.1673-4254.2025.09.15

Abstract：Objective To evaluate the photothermal and antibacterial activities of polydopamine-modified phycocyanin nanoparticles(PDA@PC NPs)and their capacity for promoting wound healing.Methods PDA@PC NPs were synthesized from phycocyanin(C-PC)and dopamine hydrochloride using a one-pot method.The photothermal activity of the nanoparticles was assessed in vitro by 808 nm laser irradiation,their biocompatibility was evaluated using CCK-8 assay,and their photothermal antibacterial activity by plate colony counting.In adult male BALB/c mice,two symmetrical full-thickness skin wounds(1.0 cm×1.0 cm)were created on both sides of the spine,and 200 μL of Staphylococcus aureus suspension was inoculated into the wounds.The mice were divided into control group,PDA@PC NPs group,and PDA@PC NPs with laser irradiation group,and wound healing rates and histomorphological changes in the wound tissues were evaluated on days 0,7 and 14 after modeling.Results The synthesized PDA@PC NPs exhibited no obvious cytotoxicity up to a concentration of 500 μg/mL and showed strong photothermal and antibacterial activities in response to 808 nm laser irradiation.In the mouse models,the size of the infected skin wounds showed substantial reduction at 7 and 14 days in PDA@PC NPs group and PDA@PC NPs with laser irradiation group,and the mean wound healing rate was faster in the latter group.HE staining and Masson's trichrome staining revealed extensive granulation tissue formation and collagen deposition on the wound surfaces in both of the treatment groups,and these changes were more obvious in the PDA@PC NPs with laser irradiation group.Conclusion PDA@PC NPs possess excellent photothermal and antibacterial activities and can effectively promote wound healing in mice.