Protective effect of Bufei Yishen Formula against cigarette smoke extract-induced human bronchial epithelial cell damage and its mechanism
FAN Zhengyuan
SHEN Zihan
LI Ya
SHEN Tingting
LI Gaofeng
LI Suyun
Abstract：Objective To evaluate the protective effect of Bufei Yishen Formula(BYF)against cigarette smoke extract(CSE)-induced injuries in human bronchial epithelial BEAS-2B cells and explore the underlying mechanism.Methods BEAS-2B cells exposed to CSE were treated with normal rat serum,BYF-medicated rat serum at low or high doses,pyrrolidine dithiocarbamate(PDTC,a NF-κB inhibitor),PDTC combined with high-dose BYF-medicated serum,or S-carbomethyloysteine(S-CMC,as the positive control).CCK-8 assay was used to determine the optimal concentration and treatment time of CSE,BYF-medicated serum and S-CMC.The treated cells were examined for inflammatory factor levels in the supernatant and cellular expressions of MUC5AC and MUC5B using ELISA,cell ultrastructural changes with transmission electron microscopy,and cell apoptosis rate using flow cytometry.The expression levels of TLR4/NF-κB pathway-associated mRNAs and proteins were determined by qRT-PCR and Western blotting.Results CSE exposure significantly increased secretions of IL-1β,IL-6 and TNF-α,mRNA and protein expressions of MUC5AC and MUC5B,and early and total apoptosis rates in BEAS-2B cells,where the presence of apoptotic bodies was detected.CSE also significantly enhanced the mRNA and protein expressions of TLR4,I-κB,and NF-κB and reduced mRNA and protein expressions of AQP5.Treatments of the CSE-exposed cells with BYF-medicated serum,PDTC and S-CMC all significantly lowered inflammatory factor levels,MUC5AC and MUC5B expressions,and early and total cell apoptosis rates,and partly reversed the changes in cellular ultrastructure and mRNA and protein expressions of the TLR4/NF-κB pathway,and the effects were the most conspicuous following the combined treatment with high-dose BYF-medicated serum and PDTC.Conclusion BYF can inhibit cell apoptosis,inflammation and mucus hypersecretion in CSE-induced BEAS-2B cells by inhibiting the TLR4/NF-κB signaling pathway.
Keywords：inflammation responsecigarette smoke extractBufei Yishen Formulamucin hypersecretionTLR4/NF-κB signaling pathway
Publication Date：2025-07-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 1372-1379 )