n-butanol fraction of ethanol extract of Periploca forrestii Schltr.:its active components,targets and pathways for treating Alcheimer's disease in rats
RAN Niandong
LIU Jie
XU Jian
ZHANG Yongping
GUO Jiangtao
Abstract：Objective To investigate the active components and possible mechanisms of n-butanol fraction of Periploca forrestii Schltr.ethanol extract for treating Alzheimer's disease(AD).Methods The active components of n-butanol fraction of Periploca forrestii Schltr.ethanol extract were analyzed using UPLC-QE-MS technique.In a SD rat model of AD induced by treatment with AlCl3 and D-gal,the therapeutic effects of low,moderate and high doses of the n-butanol fraction,saline,and donepezil hydrochloride were evaluated using ELISA,HE and Nissl staining,immunohistochemistry and Western blotting.The therapeutic mechanisms of the n-butanol fraction were explored using network pharmacology and molecular docking.Results Seventeen active components were identified from the n-butanol fraction of Periploca forrestii Schltr.ethanol extract,including phenylpropanoids,flavonoids,anthraquinones,triterpenoids,steroids,and volatile oils.In the rat models of AD,treatment with the n-butanol fraction significantly lowed AChE content in the hippocampus,increased the contents of ACh,SOD,CAT,and GSH-Px,enhanced the expressions of neuronal apoptotic factors Bcl-2,PI3K,Akt,p-PI3K,and p-Akt,and reduced the expressions of Bax and caspase-3 proteins.The treatment also dose-dependently up-regulated hippocampal expressions of Nrf-2,HO-1 and BDNF and down-regulated Keap-1,Aβ and Tau expressions.Bioinformatics analysis identified 14 key intersected targets(including TNF,AKT1 and ESR1)between the n-butanol fraction and AD.Conclusion The therapeutic effect of n-butanol fraction of Periploca forrestii Schltr.ethanol extract in AD mice is mediated by its multiple active components that regulate multiple targets and pathways.
Keywords：UPLC-QE-MSPeriploca forrestii Schltr.n-butanol extractchemical constituentsAlzheimer's diseasenetwork pharmacology
Publication Date：2025-04-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：14( 785-798 )