miRNA-128-3p inhibits malignant behavior of glioma cells by downregulating KLHDC8A expression
YU Zhengtao
LI Jiameng
JIANG Junwen
LI You
LIN Long
XIA Ying
WANG Lei
Abstract：Objective To determine whether miRNA-128-3p regulates malignant biological behavior of glioma cells by targeting KLHDC8A.Methods Dual-luciferase reporter assays,qRT-PCR and Western blotting were used to verify the targeting of miRNA-128-3p to KLHDC8A.Edu assay,flow cytometry,Transwell assay and would healing assay were used to determine the effects of changes in miRNA-128-3p and KLHDC8A expression levels on malignant behavior of glioma cells.Rescue experiment was carried out to verify that miRNA-128-3p regulated glioma cell proliferation,apoptosis,invasion and migration by targeting KLHDC8A.Results The expression level of KLHDC8A was significantly increased in high-grade glioma tissue and was closely related to a poor survival outcome of the patients.Overexpression of KLHDC8A promoted glioma cell proliferation,migration and invasion,and miRNA-128-3p overexpression inhibited proliferative and metastatic capacities of glioma cells.Mechanistically,KLHDC8A expression was directly modulated by miRNA-128-3p,which,by targeting KLHDC8A,inhibited malignant behavior of glioma cells.Conclusion Upregulation of miRNA-128-3p inhibits uncontrolled growth of glioma cells by negatively regulating KLHDC8A expression and its downstream effectors,suggesting that the miRNA-128-3p-KLHDC8A axis may serve as a potential prognostic indicator and a therapeutic target for developing new strategies for glioma treatment.
Keywords：gliomamiRNA-128-3pKLHDC8Abiomarkermigrationproliferation
Publication Date：2023-09-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：13( 1447-1459 )