rhPDCD5 suppresses pro-inflammatory cytokine secretion and proliferation and induces apoptosis of activated lymphocytes from rats with collagen-induced arthritis
WANG Wanlin
MAO Chun
XIAO Juan
Abstract：Objective To investigate the effect of recombinant human PDCD5 (rhPDCD5) treatment in a rat model of bovine II collagen (CII)-induced arthritis (CIA) on inflammatory cytokine secretion,proliferation and apoptosis of activated lymphocytes and explore the mechanisms of rhPDCD5-induced immunosuppression on activated lymphocytes.Methods Female Wistar rats were randomly divided into normal control group,CIA+ ovalbumin (OVA) group,CIA+ rhTNFR:Fc group,and CIA+rhPDCD5 group.The rats in the latter 3 groups received intraperitoneal injections of OVA (14 mg/kg),rhTNFR:Fc (3.5 mg/kg) or rhPDCD5 (14 mg/kg) from day 2 to day 26 following CII injection.On day 28,the spleens of the rats were harvested for preparing single cell suspensions of splenocytes,which were activated by CII (20 μg/mL) or anti-CD3 (1 μg/mL)+anti-CD28 (2 μg/mL) for 48 h and 72 h.The production of interferon-y (IFN-y) and interleukin-17A (IL-17A) by the activated lymphocytes was determined by ELISA of the culture supematants.The proliferation and apoptosis of the activated lymphocytes were assessed using [3H]-thymidine incorporation assay and flow cytometry,respectively.Results Compared with those in CIA +OVA group,IFN-y and IL-17A secretions by the activated lymphocytes from rhPDCD5-treated CIA rats sgnfcantly decreased.RhPDCD5 treatment of the CIA rats obvously suppressed the prolferaton and promoted apoptoss of the lymphocytes actvated by CII or by ant-CD3 + ant-CD28.Concluson rhPDCD5 reduces pro-nflammatory cytokne secreton,nhibits the proliferation and promotes activation-induced cell death of activated CD4+ lymphocytes to produce immunosuppression in rat models of CIA.
Keywords：recombinant human PDCD5lymphocytesactivation-induced cell deathrheumatoid arthritis
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 627-632 )