Intervention with Schistosoma japonicum cysteine protease inhibitor for treatment of lipopolysaccharide-induced sepsis in mice
WAN Yongkun
LI Huihui
ZUO Lin
WANG Xiaoli
WANG Liyuan
HE Wenxin
JIANG Hui
WANG Shouxiang
SHENG Jie
ZHANG Min
QIAN Haichun
YANG Fangfang
XIE Hong
GAO Shifang
FANG Qiang
YANG Xiaodi
LIU Muling
Abstract：Objective To observe the effect of Schistosoma japonicum cysteine protease inhibitor (r SjCystatin) for treatment of lipopolysaccharide(LPS)-induced sepsis in mice.Methods After a week of adaptive feeding,54 BALB/c mice were randomly divided into normal control group(group A),sepsis group(group B),and rSjCystatin intervention group(group C).The mice in group A received an intraperitoneal injection of PBS(100 μL),and those in groups B and C were injected with PBS(100 μL) containing LPS(10 mg/kg);the mice in group C were also intraperitoneally injected with 25 μg sjCystatin in 100 μL PBS 30 min after LPS injection.From each group,10 mice were randomly selected 24 h after PBS or LPS injection for detecting serum levels of TNF-α, IL-6, and IL-10 using ELISA and the levels of ALT,AST, BUN, and Cr using automatic biochemical analyzer; the pathological changes in the liver,lung and kidney were observed with HE staining.The remaining 8 mice in each group were used for observing the changes in the general condition and the 72-h survival.Results The 72-h survival rates of the mice was 100% in group A,0 in group B,and 36% in group C,showing a significant difference among the 3 groups(P<0.05).Compared with those in group A,the mice in group B exhibited obvious liver,lung,and renal pathologies with increased levels of ALT, AST, BUN, Cr, IL-6, and TNF-α (P<0.05). Treatment with sjCystatin significantly lessened LPS-induced organ pathologies, lowered the levels of liver and renal functional indexes and the pro-inflammatory cytokines,and increased the serum level of IL-10 in the mice(P<0.05).Conclusion SjCystatin can produce a significant therapeutic effect on sepsis induced by LPS in mice.
Keywords：sepsisSchistosoma japonicum cysteine protease inhibitorslipopolysaccharideproinflammatory cytokinesimmunoregulatory cytokines
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 625-629 )