miR-203 inhibits lung cancer cell metastasis by targeting fatty acid binding protein 4
CHEN Jichao
WU Xu
Abstract：Objective To explore the role of fatty acid binding protein 4 (FABP4) in regulating lung cancer cell metastasis and identify miRNAs that target FABP4.Methods The expression of FABP4 in lung cancer cells with different metastatic potentials was detected using enzyme-linked immunosorbent assay(ELISA)and Western blotting.The effects of FABP4 knockdown or overexpression by shRNA or a recombinant lentivirus, respectively, on lung cancer cells metastasis were assessed. The miRNAs that targeted FABP4 were screened using target prediction algorithms and the results were verified with Q-PCR. Results FABP4 expression was significantly higher in lung cancer cell lines with high metastatic potentials(NL9980,H661,and 95C) than in those with low metastatic potentials (L9981, A549, and PC13) (P<0.05). FABP4 knockdown in NL9980 cells resulted in significantly inhibited metastasis of the cells (P<0.05), while FABP4 overexpression obviously promoted the metastasis of A549 cells (P<0.05). The expressions of miR-203, miR-361 and miR-539 were significantly higher in highly metastatic lung cancer cells than in the cells with low metastatic potentials (P<0.05). In NL9980 cells, FABP4 expression was most obviously suppressed by miR-203(P<0.05),and target site mutational FABP4 overexpression significantly attenuated the inhibitory effect of miR-203 on NL9980 metastasis (P<0.05). Conclusion FABP4 can promote lung cancer metastasis, and by targeting FABP4 to inhibit its expression,miR-203 can suppress the metastasis of lung cancer cells.
Keywords：lung cancermetastasisfatty acid binding protein 4microRNA
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 578-583 )