Lithium chloride arrests HK-2 cell cycle in G2 phase through AKT/GSK-3β signal pathway
JIANG Tingting
ZHANG Wenying
XIANG Xiaohong
SHU Shuangshuang
XIE Wei
TANG Xun
ZHANG Jun
Abstract：Objective To investigate the effect of lithium chloride (LiCl) on cell cycle of HK-2 cells and explore the possible pathways involved.Methods HK-2 cells were treated with LiCl at different concentrations(5,12.5,20,and 25 mmol/L)for 12, 24,48,or 72 h,and the changes in cell cycle and viability were detected using flow cytometry and CCK-8 assay,respectively. Western blotting was used to analyze the changes in the expressions of cyclin B1 and CDK1 (the two G2 phase-related proteins)and those of AKT/GSK-3β signaling pathway-related proteins in the treated cells.Results LiCl treatment time-and concentration-dependently increased HK-2 cell percentage in G2 phase and decreased the cell vitality. The expressions of cyclin B1,CDK1,p-GSK-3β,and β-catenin increased and the expression of p-AKT decreased significantly in the cells as LiCl treatment time and concentration increased.Conclusion LiCl may cause HK-2 cell cycle arrest in G2 phase through activation of the AKT/GSK-3β signaling pathway.
Keywords：lithium chlorideAKT/GSK-3βHK-2 cellscell cycle
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 541-546 )