Effects of aldosterone on osteoblast proliferation, differentiation and osteogenic gene expressions in vitro
CHEN Jun
XIE Fangmei
LIN Xin
LIN Sihui
YANG Guozhu
LU Li
LU Xingyan
LI Qingnan
Abstract：Objective To study the effect of aldosterone on cell proliferation, alkaline phosphatase (AKP) activity and osteogenic gene expression in rat osteoblasts and explore the mechanisms. Methods Osteoblasts isolated from the skull of neonatal SD rats by enzyme digestion were cultured and treated with different concentrations of aldosterone. The cell proliferation and AKP activity were evaluated using CCK-8 assay kit and AKP assay kit, respectively. The effects of aldosterone on mRNA and protein expressions of the osteogenic genes and epithelial sodium channel (ENaC) gene were investigated using semi-quantitative PCR and Western blotting. Results Compared with the control cells, the cells treated with 0.01-1.0 μmol/L aldosterone showed obviously enhanced proliferation while lower (1 × 10-3 μmol/L) or higher (10 μmol/L) concentrations of aldosterone did not significantly affect the cell proliferation. Aldosterone within the concentration range of 1× 10-3 to 10 μmol/L did not cause significant changes in AKP activity in the osteoblasts. Treatment with 0.01 to 1.0 μmol/L aldosterone significantly upregulated the expressions of the osteogenic genes andα-ENaC gene at both the mRNA and protein levels. Conclusion Aldosterone within the concentration range of 0.01-1.0 μmol/L stimulates the proliferation and osteogenic gene expressions and enhances α-ENaC gene expression in rat osteoblasts in vitro, suggesting the possibility that ENaC participates in aldosterone-mediated regulation of osteoblast functions.
Keywords：aldosteroneosteoblastsosteogenic functionepithelial sodium channel
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1489-1493 )