Small interfering RNA-mediated α-enolase knockdown suppresses glycolysis and proliferation of human glioma U251 cells in vitro
LUO Qisheng
FU Huangde
HUANG Haineng
HUANG Huadong
LUO Kunxiang
LI Chuanyu
QIN Chengjian
LI Xueyu
LUO Hongcheng
WANG Junli
Tang Qianli
Abstract：Objective To investigate the role ofα-enolase (ENO1) in regulating glucose metabolism and cell growth in human glioma cells.Methods Glucose uptake and lactate generation were assessed to evaluate the changes in glucose metabolism in human glioma U251 cells with small interfering RNA (siRNA)-mediated ENO1 knockdown. MTT assay and 5-ethynyl-2'-deoxyuridine (EdU) staining were used to examine the cell growth and cell cycle changes following siRNA transfection of the cells. Results Transfection of U251 cells with siRNA-ENO1 markedly reduced glucose uptake (P=0.023) and lactate generation (P=0.007) in the cells and resulted in significant suppression of cell proliferation (*P<0.05) since the second day following the transfection. Transfection with siRNA-ENO1 also obviously suppressed cell cycle G1/S transition in the cells (P=0.0425). The expressions of HK2 and LDHA, the marker genes for glucose metabolism, were significantly down-regulated in the cells with siRNA-mediated ENO1 knockdown. Conclusion ENO1 as a potential oncogene promotes glioma cell growth by positively modulating glucose metabolism.
Keywords：α-enolasegliomasU251 cellsglucose metabolism
Publication Date：2017-01-01
Pages：5( 1484-1488 )