Structural changes of gut microbiota after gastric bypass in obese patients with type 2 diabetes
LANG Min
ZHENG Kehong
ZENG Songhua
YANG Yuan
WU Liangping
HUANG Zonghai
Abstract：Objective To investigate the structural changes inintestinal flora and metabolic changes in type 2 diabetic patients with obesity(BMI≥40 kg/m2)by sequencing the 16S rRNA genes. Methods Stool samples were collected from 4 diabetic patients before and after gastric bypass surgery for extraction of the total DNA. The diversity of the intestinal flora in the samples was investigated by 16S rRNA sequencing.After surgery,the changes in glucose and lipid metabolism were evaluated in the patients,and the changesin body mass index(BMI)and waist-to-hip ratio were assessed at 3-month intervals.Results After gastric bypass, the patient's BMI, waist-to-hip ratio, glucose metabolism and lipid metabolism gradually recovered the normal levels.The proportion of Bacteroidetesis increased and the proportions of Firmicutes and Proteobacteria decreased in the intestinal bacteria after the surgery. Conclusion Gastric bypass surgery can effectively alleviate the condition of obese patients with type 2 diabetes and improve the composition of the intestinal flora.
Keywords：16S rRNA sequence analysisintestinal floratype 2 diabetesRoux-en-Y gastric bypass
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 1337-1344 )