Expression of VEGF and PEDF in early-stage retinopathy in diabetic Macaca mulatta
CHEN Jingfei
LUO Qihui
HUANG Chao
LIU Wentao
ZENG Wen
GAO Qi
CHEN Ping
CHEN Bing
CHEN Zhengli
Abstract：Objective To explore the expression of vascular endothelial growth factor (VEGF) and pigment epithelium-derived factor (PEDF) in early stage of diabetic retinopathy (DR) in Macaca mulatta.Methods Three diabetic Macaca mulattas induced by high-fat diet were identified for early stage of diabetic retinopathy according to the fasting plasma glucose,hemoglobin Alc (HbA1c),fundus photograph and duration of diabetes,with another 3 age-matched healthy Macaca mulattas as control.The expression of VEGF and PEDF in the retinas of Macaca mulatta were detected by quantitative real-time PCR and immunohistochemistry.Result In early stage of diabetic retinopathy,VEGF mRNA and protein of the diabetic group were both significantly increased compared with the control group (P＜0.05).PEDF expression at both mRNA and protein levels was significantly decreased in diabetic Macaca mulattas compared with the control group (P＜0.01 and 0.05 respectively).Conclusion Retinal VEGF expression is increased and PEDF expression is decreased in early stage of diabetic retinopathy,suggesting their involvement in the occurrence of diabetic retinopathy and their value in assisting in the early diagnosis.
Keywords：diabetic retinopathyMacaca mulattavascular endothelial growth factorpigment epithelium-derived factor
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1217-1221 )