MACF1 knockdown in glioblastoma multiforme cells increases temozolomide-induced cytotoxicity
XIE Sidi
CHEN Ziyang
WANG Hai
HE Minyi
LU Yuntao
LEI Bingxi
LI Hezhen
LIU Yawei
QI Songtao
Abstract：Objective To investigate the role of microtubule-actin crosslinking factor 1 (MACF1) in the response of glioma cells to temozolomide (TMZ).Methods TMZ was applied to a human gliomablastoma cell line (U87) and changes in the protein expression and cellular localization were determined with Western blot,RT-PCR,and immunofluorescence.The responses of the cells with MACF1 expression knockdown by RNA interference to TMZ were assessed.TMZ-induced effects on MACF1 expression were also assessed by immunohistochemistry in a nude mouse model bearing human glioblastoma xenografts.Results TMZ resulted in significantly increased MACF1 expression (by about 2 folds) and changes in its localization in the gliomablastoma cells both in vitro and in vivo (P＜0.01).Knockdown of MACF1 reduced the proliferation (by 45％) of human glioma cell lines treated with TMZ (P＜0.01).TMZ-induced changes in MACF1 expression was accompanied by cytoskeletal rearrangement.Conclusion MACF1 may be a potential therapeutic target for glioblastoma.
Keywords：cytoskeleton rearrangementmicrotubule-actin crosslinking factor 1temozolomideglioblastoma multiforme
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 1183-1189 )