Effect of macrophage inflammatory protein-1β on proliferation and apoptosis of human tongue squamous cell carcinoma CAL-27 cells in vitro
JIA Bo
QIU Xiaoling
CHU Hongxing
SUN Xiang
PAN Jie
WANG Zhiping
ZHAO Jianjiang
Abstract：Objective To detect CCR5 protein expression in different human tongue squamous cell carcinoma cells and observe the effect of macrophage inflammatory protein-1β (MIP-1β) on the proliferation and apoptosis of CAL-27 cells. Methods Western blotting and immunofluorescence staining were used to detect the expression of the CCR5, the receptor of MIP-1β, in 3 human tongue squamous cell carcinoma cells UM-1, CAL-27, and Tca-8113. CCK-8 assay was used to assess the proliferation of CAL-27 cells stimulated with 10, 20, and 40 ng/mL MIP-1β for 12, 24, or 48 h. The apoptosis of the cells stimulated with MIP-1β(10, 20, and 40 ng/mL) for 24 h was analyzed using flow cytometry with Annexin V/PI double staining. Results CCR5 expression was detected both on the membrane and in the cytoplasm in all the 3 tongue squamous cell carcinoma cell lines. At the concentrations of 10, 20, and 40 ng/mL, MIP-1β stimulation for 12 and 24 h significantly promoted the proliferation of CAL-27 cells (P<0.05);MIP-1βstimulation for 48 h at the concentrations 10 and 20 ng/mL, but not at 40 ng/mL, promoted the proliferation of CAL-27 cells (P<0.05). MIP-1βstimulation at 40 ng/mL for 24 produced the most obvious apoptosis-inducing effect in CAL-27 cells (P<0.05), while MIP-1β at 10 or 20 ng/mL did not induce obvious apoptosis in the cells (P>0.05). Conclusion CCR5 is expressed in all the 3 human tongue squamous cell carcinoma cells. MIP-1βcan promote the proliferation of CAL-27 cells but high concentrations of MIP-1βalso induced cell apoptosis. Prolonged stimulation of the cells with a high concentration of MIP-1βshows attenuated effect in promoting cell proliferation probably as a result of cell apoptosis induced by MIP-1β.
Keywords：tongue cancerchemokineproliferationapoptosismacrophage inflammatory protein-1β
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1104-1109 )