The MicroRNA miR-205 inhibits epithelial-messenchymal transition in HK-2 cells by down-regulating ZEB1 and ZEB2 expressions
CAO Luoyuan
YANG Jing
FU Xianguo
LIN Yinghua
LIN Feng
HUANG Baoying
Abstract：Objective To explore the role of miR-205 in regulating epithelial-messenchymal transition (EMT) in proximal tubular cell line HK-2 cells and the underlying mechanism.Methods HK-2 cells transfected with miR-205 mimics or a scrambled control sequence were examined for miR-205 expressions and mRNA levels of ZEB1,E-cadherin,and α-SMA using real-time qPCR;the protein levels of ZEB1,ZEB2,E-cadherin,and α-SMA were detected with Western blotting.Immunohistochemistry was performed to examine the ectopic expression of β-catenin and E-cadherin expression in the cells.Results The expression levels of ZEB1 and ZEB2 decreased significantly (P＜0.01) while E-cadherin expression was up-regulated (P＜0.01) in cells transfected with miR-205 mimics.Transfection with miR-205 mimics also markedly down-regulated the expression of α-SMA (P＜0.01),a marker of mesenchymal cells that play an important role in EMT of HK-2 cells.The ectopic expression of β-catenin was inhibited by miR-205 mimics in HK-2 cells.Conclusion miR-205 inhibits EMT in HK-2 cells by down-regulating the expression levels of ZEB1 and ZEB2.
Keywords：miR-205epithelial-messenchymal transitionHK-2 cellsZEB1
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 1700-1705,1711 )