Interaction of DNAJB13 with HK1 in mouse
YANG Mengyue
XIONG Ziwei
LI Weina
JIA Miaomiao
LIU Gang
Abstract：Objective To investigate the presence of interactions between DNAJB13 and HK1.Method The open reading frame of Dnajb13 gene was amplified from mouse testis cDNA by PCR.The PCR products were then inserted into pGEX-4T-1 vector after double digestion and identified by sequancing.The recombinant plasmids were transformated into competent DH5a cells,and the fusion protein was expressed with IPTG induction.SDS-PAGE Coomassie brilliant blue staining and Western blot analysis were used to detect the fusion protein expression.The protein precipitated by GST-DNAJB13 in GST pull down assay was detected by Western blotting.Results The recombinant plasmid pGEX-4T-1-Dnajb13 was successfully constructed and verified.E.coli transformed with the recombinant plasmid expressed abundant fusion protein.GST pull down assay showed interactions between DNAJB13 and HK1.Conclusion DNAJB13 interacts with HK1 in mouse testis and probably participates in spermatogenesis and the regulation of sperm motility.
Keywords：DNAJB13recombinantplasmidfusion proteinHK1GST pull downspermatogenesissperm motility
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1684-1688 )