Expression of mir-106b in esophageal squamous cell carcinoma
XIAO Changyan
GUO Zijian
CAO Lei
DING Xiaowen
LIU Qiang
WANG Xiaochun
Abstract：Objective To investigate the expression of mir-106b in esophageal squamous cell carcinoma (ESCC) tissue and analyze its correlation with the cilnicopathologic features of ESCC.Methods A total of 200 fresh surgical specimens of ESCC and adjacent tissues collected between 2001 and 2007 were examined for expressions of mir-106b using real-time PCR (RT-PCR).Northern blot analysis for mir-106b was performed in 4 pairs of samples to confirm the RT-PCR results.The relationship between mir-106b expression and clinicopathological features and prognosis of the patients were analyzed.Results Mir-106b was expressed at significantly higher levels in ESCC tissues than in the paired adjacent tissues.Overexpression of mir-106b was associated with lymph node metastasis,stage of TNM classification and smoking (P＜0.05).The survival rate of patients with low mir-106b expression was higher than that of patients with high mir-106b expression (60 vs 37 months,P=0.024).Cox regression analysis indicated that the expression of mir-106b,lymph node metastasis and smoking were independent prognostic factors for ESCC (P＜0.05).Conclusion Mir-106b is overexpressed in ESCC tumors,and its overexpression is strongly associated with lymph node metastasis and a poor prognosis.Mir-106b expression is an independent prognostic factor for ESCC and can serve as a biomarker for diagnosis and prognostic evaluation of ESCC.
Keywords：mir-106boverexpressionprognosisesophageal squamous cell carcinoma
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1667-1671 )