Oestrogen inhibits invasion and metastasis of hepatocellular carcinoma MHCC97H cells by regulating the activity of AKT signaling pathway
TIAN Changyin
ZHANG Xin
ZHAO Wenxue
DANG Shuangsuo
JIN Yaofeng
JI Fanpu
Abstract：Objective To explore the inhibitory effect of estrogen against metastasis of human hepatocellular carcinoma MHCC97H cells and explore the molecular mechanism.Methods The inhibitory effect of estrogen on the migration and invasion of MHCC97H cells was evaluated with wound healing assay and Transwell assay.Western blotting was used for investigating the expression of MMP-2,MMP-9,AKT and p-AKT in the cells treated with estrogen.Results Estrogen treatment significantly inhibited the migration and invasion of MHCC97H cells in a dose-dependent manner.Estrogen significantly down-regulated the protein expressions of MMP-2 and MMP-9 and lowered the phosphorylation level of AKT.Conclusion The anti-metastatic effect of estrogen involves inhibition of MMP-2 and MMP-9 in MHCC97H cells probably by regulating AKT signal pathway.
Keywords：estrogenMHCC97H cellsinvasionAKT signal pathwaymetalloproteinases
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1621-1625 )