Effects of bisoprolol pretreatment on hypoxia/reoxygenation-induced injury in H9c2 cardiomyocytes
XI Mingming
LIU Jing
XIE Liang
GONG Jianbin
Abstract：Objective To investigated the effects of bisoprolol pretreatment on hypoxia/reoxygenation (H/R)-induced injury in H9c2 cardiomyocytes. Methods Cultured H9c2 cells were exposed to hypoxia for 6 h followed by reoxygenation for 2 h with or without pretreatments with bisoprolol or bisoprolol+LY294002. The cell survival was measured by MTT assay, and the cell apoptosis and levels of radical oxygen species (ROS) were evaluated with flow cytometry. The protein levels of phosphyorylated AKT and phosphorylated GSK3βin the cells were determined by Western blotting. Results Compared with the normal control cells, the cells exposed to H/R injury showed significantly decreased cell survival and increased cell apoptosis and ROS production; pretreatment of the cells with bisoprolol significantly decreased the cell apoptotic rates and ROS production and obviously enhanced the cell survival and protein levels of p-AKT and p-GSK3βin the exposed cells. The protective effect of bsioprolol against H/R-induced cell injury was significantly attenuated by LY294002. Conclusion Bisoprolol can protect H9c2 cells against H/R-induced injury and oxidative stress by activating PI3K/AKT/Gsk-3βpathway to increase the phosphorylation of AKT and GSK3βand reduce ROS production.
Keywords：bisoprololhypoxia/reoxygenation injuryH9c2myocardial protectionPI3K/AKTGSK3β
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1198-1203 )