miR-124 regulates radiosensitivity of colorectal cancer cells by targeting PRRX1
LIN Shuimiao
XIA Qiong
ZHANG Yuqin
SUN Aimin
SHI Yusheng
ZHENG Lin
CHEN Longhua
Abstract：Objective To detect the expression of miR-124 in colorectal carcinoma (CRC) cells and tissue specimens and analyze its association with the radiosensitivity of the cells. Methods The expression of miR-124 in CRC cell lines and tissues were detected using qRT-PCR. The effect of miR-124 in modulating cell radiosensitivity was assessed in CRC cells with miRNA-124 overexpression and miRNA- 124 knockdown, and bioinformatics prediction and dual luciferase reporter system were employed to identify the direct target of miR-124. Results miR-124 expression was down-regulated in CRC cell lines and tissues. CRC cells over-expressing miR-124 showed an obviously enhanced radiosensitivity, whereas miR-124 knockdown resulted in a reduced radiosensitivity of the cells. Bioinformatics prediction and dual luciferase reporter system verified PRRX1 as a direct target of miR-124, which regulated the radiosensitivity of CRC cells by directly inhibiting PRRX1. Conclusion miR-124 can enhance the radiosensitivity of CRC cells by directly targeting PRRX1, which provides a target for improving the therapeutic effect of radiotherapy of CRC.
Keywords：colorectalcancermiR-124radiosensitivityPRRX1
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 1110-1116 )