ApoE4 increases glycogen synthase kinase 3βexpression and Tau phosphorylation in U87 cells
HE Yanjie
WEI Peiru
WU Qiaoyan
ZHANG Xinyu
ZHANG Xingmei
LIU Xiaojia
WANG Fang
Abstract：Objective To explore the relations among apolipoprotein E4, Tau protein and glycogen synthase kinase 3β(GSK-3β). Methods U87 cells were transfected with pIRES-EGFP (control) or the recombinant plasmids ApoE4/pIRES-EGFP or ApoE3/pIRES-EGFP, and the expression levels of p-Tau/Tau and GSK-3βin the cells were examined with Western blotting. To further confirm the effect of ApoE on GSK-3βand p-Tau expressions, a short interfering RNA (siRNA) targeting ApoE (ApoE-siRNA) was transfected into U87 cells via Lipofectamine 2000 and the protein expressions were examined 24 h later. Results Compared with those in the control group, the expressions levels of both GSK-3β and p-Tau/Tau increased significantly in the cells transfected with ApoE4 and ApoE3 plasmids (P<0.01), and the ApoE4 plasmid produced a more potent effect than the ApoE3 plasmid on the protein expressions (P<0.01). ApoE knockdown resulted in significantly reduced expressions of GSK-3β (P<0.001) and p-Tau (P<0.01) in the cells. Conclusion ApoE4 can enhance Tau phosphorylation though upregulating GSK-3β, which sheds light on a new role of ApoE4 in Alzheimer's disease.
Keywords：ApoEAlzheimer's diseaseTauglycogen synthase kinase 3β
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 904-908 )