Diallyl disulfide inhibits invasion and metastasis of MCF-7 breast cancer cells in vitro by down-regulating p38 activity
CHEN Xiaoxiao
LIU Xiaowang
ZHOU Zhigang
CHEN Xueyan
LI Liding
XIONG Ting
PENG Lu
TU Jian
Abstract：Objective To investigate the effect of diallyl disulfide (DADS) on invasion and metastasis of human breast cancer MCF-7 cells and explore the possible mechanism. Methods MCF-7 cells treated with 100, 200, and 400μmol/L of DADS for 24 h were examined for cell invasion and migration capacities using Transwell assay and wound healing assay, respectively. The protein expression of E-cadherin, vimentin, MMP-9 and p-p38 in the cells were detected with Western blotting. The effect of transforming growth factor-β1 (TGF-β1) as the agonist of p38 activity was tested in antagonizing the effects of DADS. Results DADS inhibited the invasion and migration of MCF-7 cells in a dose-dependent manner, down-regulated the protein expression of Vimentin and MMP-9 and up-regulated E-cadherin expression in the cells. Treatment with TGF-β1 to up-regulate p38 activity obviously antagonized the inhibitory effect of DADS on the invasion and metastasis of MCF-7 cells. Conclusion DADS can inhibit the invasion and metastasis of MCF-7 cells in vitro by down-regulating p38 activity.
Keywords：diallyl disulfideMCF-7 breast cancer cellsp38 activityinvasionmetastasis
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 814-818 )