Nanog promotes the invasion of breast cancer cells by increasing PKC?expression
SHAO Wenfeng
LI Fuxi
TANG Rui
YU Xiaoran
WEN Qiaosheng
YU Yanlin
XIONG Jingbo
Abstract：Objective To study the relationship between Nanog- promoted metastasis of breast cancer and ezrinT567 phosphorylation, and explore the possible mechanism by which Nanog regulates ezrinT567 phosphorylation. Methods A siRNA construct targeting Nanog was transfected in breast cancer cells to knock down Nanog expression, and the changes in the cell invasion was detected using Transwell assay. The expression levels of Nanog and PKC and the phosphorylation level of ezrinT567 were detected using Western blotting and immunofluorescent staining; the protein interaction between PKC? and ezrin was assayed by co-immunoprecipitation and Western blotting. Results Nanog knockdown significantly decreased the expression of PKC?protein, phosphorylation level of ezrinT567 and the invasion ability of breast cancer cells. PKCεknockdown obviously decreased the phosphorylation level of ezrinT567 in the cells, and PKC? and ezrin were co-immunoprecipitated. Concludions Nanogcan can upregulate the expression of PKC? to promote the phosphorylation of ezrinT567, which can be a new mechanism by which Nanog promotes tumor metastasis.
Keywords：Nanogezrinprotein kinase Cbreast cancerinvasion
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 639-644 )