TALEN-mediated MYH9 Knock-down and its influence on cell cycle and apoptosis of MGC803 cell line
ZHU Xianjun
DENG Haijun
YE Gengtai
SHEN Zhiyong
LI Fengping
GUO Weihong
YANG Qingbin
LIU Hao
LI Guoxin
Abstract：Objective To construct a MYH9 gene knockout model in MGC803 cell line using transcription activator-like effector nuclease (TALEN) and observe its effect on cell cycle and apoptosis. Methods According to FastTALETM TALEN Kit, we designed TALEN pairs and constructed the plasmids targeting to MYH9 gene. After detecting their activity in MGC803 cells by plasmid transfection, DNA sequencing, RT-PCR and western blot, we selected the monoclonal cells and studied the changes in the cell cycle and apoptosis. Results MYH9 gene could not be knocked out but knocked down in selected MGC803 monoclonal cells, which caused cell cycle arrested at G2/M phase (P<0. 05) and a significant increase in the cell number with early apoptosis (P<0. 01). Conclusion We successfully generated a MYH9 knockdown model in MGC803 cell lines by TALEN, which could be in favor of MYH9 function study in gastric cancer.
Keywords：transcription activator-like effector nucleaseMYH9 genecell cyclecell apoptosis
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 375-380 )