Effects of exendin-4 on extracellular matrix metabolism in human mesangial cells cultured in high glucose
XIAO Zhizhou
GUAN Meiping
ZHENG Zongji
JIA Yijie
WANG Ling
XUE Yaoming
Abstract：Objective To explore effects of exendin-4 on the metabolism of extracellular matrix (ECM) in human mesangial cells (HMC) cultured in the presence of high glucose and explore the possible mechanism. Methods Human mesangial cells (HMC) were treated with exendin-4 under high glucose conditions. The cell proliferation was observed using CCK8 assay, and the expressions of collagen type I, fibronectin, transforming growth factor-β1 (TGFβ1) expression and extracellular signal-regulated kinase (ERK) signaling pathway activity were assessed using Western blotting. Results Exendin-4 inhibited cell proliferation and the expressions of collagen type I, fibronectin and TGFβ1 and reversed ERK phosphorylation in high glucose-induced HMC. Conclusion Exendin-4 can regulate ECM metabolism in HMC cultured in high glucose by inhibiting TGFβ1/ERK pathway, suggesting the beneficial effects of exendin-4 in preventing and treating diabetic nephropathy.
Keywords：exendin-4human mesangial cellextracellular matrixhigh glucose
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 371-374 )