Effect of the hydrophobin HFBI-fusion tag on exogenous protein accumulation in tobacco plant
ZHANG Xiqian
MU Hongzhen
MA Ting
DING Xiangzhen
LI Zhiying
WANG Sheng
Abstract：Objective To explore the mechanisms by which HFBI fusions increase recombinant fusion protein accumulation in plants. Methods The HFBI sequence from Trichoderma reesei was synthesized and two plant expression vectors for expression of green fluorescence protein (GFP) and GFP-HFBI were constructed. The vectors were inoculated in Nicotiana benthamiana plants through agroinfiltration, and the expression levels and mRNA accumulation levels of GFP in Nicotiana leaves were examined by Western blotting, ELISA and RT-PCR. Results The HFBI fusion tag significantly enhanced the accumulation of GFP in the leaves of N. benthamiana without causing toxic effects. Endoplasmic reticulum-targeted GFP-HFBI fusion induced the formation of spherical protein particles in the plant cells. Conclusion HFBI fusions can increase the accumulation of its fusion partner in plants by forming stable protein particles, which probably shields the target protein from endogenous protease-induced degadation. HFBI fusion technology provides an alternative to improving recombinant protein expression in plants from agroinfection-compatible expression vectors.
Keywords：hydrophobinfusion tagsgreen fluorescent proteinTrichoderma reeseiNicotiana benthamiana
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 1665-1671 )