Minocycline reduces hyperpolarization-activated current in rat substantia gelatinosa neurons
ZHU Mengye
LIU Nana
PENG Sicong
LI Lingchao
ZHANG Daying
LIU Tao
Abstract：Objective To investigate the effect of minocycline on hyperpolarization-activated current (Ih) in the substantia gelatinosa (SG) neurons in rat spinal dorsal horn. Methods In vitro spinal cord transverse slices were prepared from 3-5-week-old male Sprague-Dawley rats. Using whole-cell patch clamp technique, Ih currents were recorded before and after bath application of minocycline (1-300 μmol/L) to the SG neurons. Results Ih currents were observed in nearly 50% of the recorded neurons, and were blocked by Ih blocker CsCl and ZD7288. Minocycline rapidly and reversibly reduced the amplitude of Ih and decreased the current density in a concentration-dependent manner with an IC50 of 34μmol/L. Conclusion Minocycline suppresses the excitability of SG neurons through inhibiting the amplitude and current density of Ih and thereby contributes to pain modulation.
Keywords：minocyclinehyperpolarization-activated currentsubstantia gelatinosa
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 1155-1161 )