Effect of indomethacin on BCR/ABL-Wnt/β-catenin pathway in K562 cells
LIU Zhangling
HU Jing
HUANG Zhenglan
LI Hui
LIU Xin
FENG Wenli
Abstract：Objective To investigate the effect of indomethacin on the proliferation and Wnt/β-catenin pathway in K562 cells. Methods The cell growth of K562 cells treated with different concentrations of indomethacin was assessed with MTT assay, and the colony-forming ability of the cells was evaluated by colony-forming assay. The mRNA expressions of BCR/ABL andβ-catenin were detected by RT-PCR, and the protein expressions of pBCR/ABL, total BCR/ABL,β-catenin, pGSK-3βand c-myc were analyzed by Western blotting. Results Indomethacin significantly suppressed the growth and colony-forming ability of K562 cells in a dose-dependent manner. Indomethacin treatment dose-dependently decreased the protein level of pBCR/ABL and total BCR/ABL without affecting bcr-abl mRNA expressions. Compared with the control groups, indomethacin-treated cells showed obviously decreased mRNA and protein expressions ofβ-catenin and decreased protein expressions of pGSK-3βand c-myc. Conclusion Indomethacin inhibits the proliferation of K562 cells by suppressing the activity of bcr-abl-Wnt/β-catenin pathway.
Keywords：indomethacinchronic myelogenous leukemiaK562 cellsBCR/ABLWnt/β-cateninpathway
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 998-1002 )