Expression and functional assessment of solute carrier 26A transporter family in HEK-293 cells
FAN Chao
FU Mingyu
XIAO Zhongju
TANG Jie
Abstract：Objective To express solute carrier 26A proteins in HEK-293 cells and explore their functions. Methods SLC26A-eGFP plasmids were transiently transfected into HEK-293 cells, and the nonlinear capacitance of the cells expressing SLC26A proteins was measured by whole-cell patch recording. Results All the SLC26A transporters were expressed on the membrane of HEK-293 cells. Each member of the SLC26A transporter family showed robust nonlinear capacitance, which represented their binding capability with anions. Conclusion The SLC26A transporters expressed on HEK cells show similar functions as expected in tissue environment. The plasmids we constructed facilitate structural and functional study of SLC26A transporters.
Keywords：human embryonic kidney 293 cellssolute carrier protein 26Awhole-cell patch recordingnonlinear capacitance
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 801-806,811 )