Observation of radiobiological characteristics in a HepG2 cell line with mitochondrial DNA deletion
SUN Hengwen
PAN Yi
ZENG Zijun
FANG Liangyi
ZHANG Hongdan
XIE Songxi
LI Weixiong
XU Jiabin
Abstract：Objective To study the radiobiological characteristics of a HepG2 cell line with mitochondrial DNA (mtDNA) deletion. Methods HepG2 cells were cultured in a medium containing ethidium bromide, acetylformic acid and uracil. The HepG2 cell line with mtDNA deletion (ρ0HepG2 cells) were acquired after 30 subcultures by limited dilution cloning. The cell survival was then observed in the absence of acetylformic acid and uracil, and the total mtDNA deletion in the cells was confirmed by PCR. The radiosensitivity of HepG2 andρ0HepG2 cells was evaluated by exposure to gradient doses of 6 MV X ray irradiation. The cell apoptosis was assessed following a 2 Gy X-ray exposure with Hochest33342 staining, and the invasiveness of ρ0HepG2 cells was measured by Transwell assay. Results HepG2 cells could survive 30 subcultures in the presence of ethidium bromide, and massive cell death occurred after removal of acetylformic acid and uracil from the medium. PCR confirmed total mtDNA deletion fromρ0HepG2 cells, whoseα/βvalue was significantly lower than that of HepG2 cells.ρ0Hep-G2 cells showed an obviously lowered cell apoptosis rate following X-ray exposure with enhanced cell invasiveness. Conclusion HepG2 cells can be induced by ethidium bromide intoρ0HepG2 cells with an increased radiation resistance, anti-apoptosis ability and cell invasiveness.
Keywords：radiosensitivityHepG2Rho0 cellsapoptosis
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 783-788 )