Changes in splenocyte proliferation, subsets and cytokine production in mice immunized with recombinant vaccine Bifidobacterium bifidum (pGEX-Sj32) of Schistosoma japonicum
TAN Jianrong
LI Wengui
QIN Ting
Abstract：Objective To observe the dynamic changes of immune responses of splenocytes in mice immunized with recombinant vaccine Bifidobacterium bifidum (pGEX- Sj32) of Schistosoma japonicum and investigate the immunological mechanism of the vaccine. Methods Eighty-eight BALB/c mice were randomized for immunization with 106 CFU recombinant vaccine orally or with 105 CFU recombinant vaccine intranasally. Four mice were selected from each group every two weeks to test the responses of the splenocytes to stimulations with SjAWA or ConA. MTT assay and flow cytometry were used to assess splenocyte proliferation and the distribution of CD4+and CD8+T cells, respectively;the levels of interleukin-10 (IL-10), IL-12 and tumor necrosis factor-α (TNF-α) in the cell culture supernatant were detected by ELISA. Results Regardless of the stimulations, the splencytes showed significantly enhanced proliferation in weeks 2-16 in oral administration group and in weeks 2-18 in intranasal group (P<0.01). CD4+subsets in both two groups increased obviously in weeks 2-12 (P<0.01) but CD8+subsets remained stable. In oral administration group, the levels of TNF-α, IL-10 and IL-12 increased in weeks 2-14, 2-18 and 2-14, and peaked at week 8, 10 and 6, respectively;in intranasal group, the cytokines increased in weeks 2-14, 2-18 and 2-18, and peaked at week 8, 10 and 8, respectively. Conclusion The recombinant vaccine rBb (pGEX-Sj32) can induce effective immune responses in mice.
Keywords：Schistosoma japonicumrBb(pGEX-Sj32) vaccinesplenocyteproliferationT cell subsetscytokines
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 202-207 )