Tetramethoxystilbene, a selective CYP1B1 inhibitor, suppresses adipogenesis of C3H10T1/2 pluripotent stem cells
FAN Cuifang
ZHU Anna
HUANG Tingting
LI Lu
WANG Suqing
Abstract：Objective To investigate the inhibitory effects of tetramethoxystilbene, a selective CYP1B1 inhibitor, on adipogenic differentiation of C3H10T1/2 multi-potent mesenchymal cells. Methods In vitro cultured C3H10T1/2 cells at full confluence were induced by adipogenic agents (10μg/ml insulin, 2μmol/L dexamethasone and 0.5 mmol/L 3-isobutyl-1-methylxanthine) and exposed simultaneously to TMS at the final concentrations of 1.0, 2.0 or 4.0μg/ml. Oil Red-O staining was used to observe the cell differentiation. The expression of peroxisome proliferator-activated receptor gamma (PPARγ) and its target genes cluster of differentiation 36 (CD36) and fatty acid binding protein 4 (FABP4) were quantified by real-time RT-PCR and Western blotting. Results Oil Red-O staining and TG contents revealed that TMS suppressed induced differentiation of C3H10T1/2 cells. TMS exposure of the cells dose-dependently decreased both mRNA and protein expressions of PPARγ, a key nuclear transcription factor during adipogenesis, and also lowered the mRNA expressions of PPARγ target genes CD36 and FABP4. Conclusion TMS can suppress adipogenic differentiation of C3H10T1/2 cells by inhibiting PPARγ.
Keywords：tetramethoxystilbeneCYP1B1 inhibitorC3H10T 1/2 cellsperoxisome proliferator-activated receptor gammaadipogenesis
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 72-76 )