Neural stem cell-specific peroxisome proliferator-activated receptorγknockout mice:breed-ing and genetic identification
WU Qiaoqi
ZHANG Hongyan
WANG Zhen
LIN Lifang
CHEN Lu
WANG Xuemin
Abstract：Objective To breed neual stem cell-specific peroxisome proliferator-activated receptor γ (PPARγ) knockout mice. Methods Two transgenic mouse models, namely B6.PPARγloxp/loxp and B6.Nestin-Cre were interbred, and the first-generation offsprings were backcrossed with B6.PPARγloxp/loxp to obtain the second-generation mice. Genomic DNA was extracted from the second-generation mice for PCR to amplify the loxp and Cre gene fragments followed by agarose gel electrophoresis to verify their sizes. The mice with the PPARγloxp/loxp.Nestin-Cre (KO) genotype were selected as the neural stem cell-specific knockout PPARγ mice, with B6.PPARγloxp/loxp (loxp) mice as the control. Tissue samples were collected from specific regions of the mouse brain and peripheral tissue for detecting the expression of PPARγ mRNA using RT-PCR and real-time quantitative PCR. Results and Conclusion Genotyping results showed PPARγloxp and Cre bands in the knockout mice, which showed obviously decreased mRNA expression of PPARγ, suggesting successful establishment of neural stem cell-specific PPARγ knockout mice. The two transgenic mice we used were fertile, and their breeding pattern followed the laws of Mendelian inheritance.
Keywords：peroxisome proliferator-activated receptorγgene knockoutCre-loxp
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1768-1771 )