Effects of Biejiajian Pills on Wnt signal pathway signal moleculesβ-catenin/TCF4 complex activities and downstream proteins cyclin D1 and MMP-2 in hepatocellular carcinoma cells
WEN Bin
SUN Haitao
HE Songqi
CHENG Yang
JIA Wenyan
FAN Eryan
PANG Jie
Abstract：Objective To study the effect of Biejiajian Pills on Wnt signal pathway and the mechanisms underlying its action to suppress the invasiveness of hepatocellular carcinoma. Methods HepG2 cells cultured in the serum of rats fed with Biejiajian Pills for 48 h were examined for β-catenin expression using immunofluorescence, β-catenin/TCF4 complex activity with luciferase, and expressions of the downstream proteins cyclin D1 and MMP-2 using qRT-PCR. Results Biejiajian Pills-treated sera significantly reduced the expressions of cytoplasmic and nuclear β-catenin protein, cyclin D1 and MMP-2 proteins and lowered the activities ofβ-catenin/TCF4 complex. Conclusion Biejiajian Pills may serve as a potential anti-tumor agent, whose effect might be mediated by inhibiting the Wnt/β-catenin pathway.
Keywords：hepatocellular carcinomaBiejiajian Pillsβ-catenin/TCF4 complexcyclin D1MMP-2
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1758-1762 )