Alternative polyadenylation site analysis of tumor-related genes based on 3'RACE in gastric cancer cells
LAI Dengpan
CHEN Jian
KANG Yani
Abstract：Objective To analyze the alteration in alternative polyadenylation (APA) sites of tumor-related genes in gastric cancer cells. Methods We used 3'RACE to capture the APA sites of two tumor-related genes (HSP90αand SEC11A) in gastric cancer cell lines MKN45, MKN28 and AGS, and compared the results with annotated poly(A) sites in UCSC database. Results We found new APA sites in the two tumor-related genes in gastric cancer cells to produce new mRNA isoforms with different 3'UTRs. Conclusions There are new mRNA isoforms of HSP90αand SEC11A derived from ATA in gastric cancer cells, which provides new insights into the mechanisms of gastric tumorigenesis.
Keywords：gastric cancer3'RACEalternative polyadenylation
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1738-1742 )