Live cell fluorescent imaging and cytotoxicity assessment of pH fluorescent probe based on styrylcyanine dyes
HUANG Lixia
LIU Xiang
CUI Danting
XU Yikai
YOU Wenwei
YAN Yichen
LU Xinwei
LIU Ruiyuan
Abstract：Objective To prepare a pH fluorescence probe based on styrylcyanine dyes for live cell imaging. Methods The Probe 1 was prepared by reaction of 4-pyridinecarboxaldehyde with 1,1,2-trimethylbenz[e]indole. The influence of pH on the fluorescent properties was examined, and the cell viability was examined using cell counting kit-8. The Probe 1 was used as a pH fluorescence probe in living cell. Results Probe 1 emitted green fluorescence under neutral and basic conditions but orange fluorescence under acid condition. Probe 1 selectively stained the cytoplasmic regions of living cells without significantly affecting the cell viability. Conclusion The pH-sensitive fluorescent probe prepared based on styrylcyanine possesses good ability of cell membrane permeation for live cell fluorescent imaging.
Keywords：styrylcyaninepH fluorescent probecell imagingcytotoxicity
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1642-1645 )