Association of vitamin D receptor Fok I and Bsm I polymorphisms with dyslipidemias in el-derly male patients with type 2 diabetes

XIA Zheng; HU Yazhuo; ZHANG Honghong; HAN Zhitao; BAI Jie; FU Shuhong; DENG Xinli; HE Yao

doi:10.3969/j.issn.1673-4254.2014.11.02

Abstract：Objective To assess the association of vitamin D receptor (VDR) gene Fok I and Bsm I polymorphisms with dyslipidemia in elderly male patients with type 2 diabetes of Han nationality. Methods A total of 328 elderly male residents of Han nationality in Beijing, including 237 type 2 diabetic patients and 91 healthy control subjects, were enrolled in this study. The diabetic patients were divided into non-dyslipidemia group (DO group, n=134) and dyslipidemia group (DH group, n=103). All the participants were genotyped for Fok I and Bsm I polymorphisms in VDR gene using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing technology, and the results were compared with their clinical characteristics. Results For Fok I, the frequency of F allele was significantly higher in the diabetic patients than in the control group (χ2=3.873, P=0.049, OR=1.439, 95%CI:1.001-2.071). In the dominant model, the frequency of FF genotype was significantly higher in the diabetic group (χ2=5.057, P=0.025, OR=1.756, 95%CI:1.072-2.875) as well as in DH group (χ2=6.168, P=0.013, OR=2.06, 95%CI:1.161-3.663) than in the control group. There was no significant differences in the genotype frequency or allele distribution in other paired groups (P>0.05). Compared with Ff + ff genotype, FF genotype was associated with a significantly decreased average diastolic blood pressure (P=0.039) but significantly increased postprandial blood glucose (P=0.035), triglycerides (P=0.049) and uric acid (P=0.031). No significant difference was detected in genotype frequency or allele distribution of Bsm I polymorphisms between the groups (P>0.05); serum creatinine levels were significantly higher in bb genotype than in BB + Bb genotype group (P=0.011). Conclusion VDR gene Fok I polymorphisms may be a risk factor for dyslipidemia in elderly male patients with type 2 diabetes among Chinese Han population, where Bsm I polymorphisms are not associated with diabetic dyslipdiemia.