Ethanol reduces neural precursor cells and inhibits neuronal and glial differentiation in zebrafish embryos
YIN Guo
YAO Fang
CHEN Xiaohui
WANG Nan
WANG Huili
CHANG Hongen
YUAN Zhaohu
WU Bingyi
Abstract：Objective To investigate the influence of exposure to different concentrations of ethanol on neural progenitor cells and the differentiation of neurons and glial cells in zebrafish embryos. Methods Zebrafish embryos were exposed to 1%, 2%, and 2.5%(V/V) ethanol at 5 hpf by adding ethanol to the egg water. In situ hybridization and real-time PCR were used to detect the changes in the mRNA expression profiles of the markers of different cells to examine the effects of alcohol on neural development. Results The number of neural precursor cells, neurons and mature glial cells was significantly reduced in the zebrafish embryos following ethanol exposure, and this reduction became more prominent as the ethanol concentration increased. The expression of the early glial marker slc1a3a was down-regulated in the spinal cord but increased in the brain after exposure to increased ethanol concentrations. The expression of the mature glial markers was significantly lowered in response to exposure to increasing ethanol concentrations. Conclusion Ethanol can reduce neural precursor cells and inhibits neuronal and glial differentiation in zebrafish embryos.
Keywords：zebrafishneural progenitor cellsglial cellsneuronsdifferentiation
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 1555-1561 )