Starvation-induced autophagy in cultured non-small cell lung cancer cells
GUO Qianqian
LIU Zhiyan
JIANG Lili
HU Tinghua
LI Dongfan
LIU Ying
LIU Mengjie
LIANG Xuan
NAN Kejun
Abstract：Objective To observe autophagy induced by starvation in non-small cell lung cancer A459 and 95D cells. Methods A549 and 95D cells in logarithmic growth in 1640 medium were cultured in Earle's balanced salt solution (EBSS) for 0, 1, 2, 3, 4 or 5 h. Autophagosome formation in the cell culture was observed by MDC fluorescent staining, and the expression of microtubule-associated protein 1 light chain 3 (LC3) and Beclin1 in the cells were detected using Western blotting. Results Compared with the control cells, the cells with prolonged starvation showed increased MDC- positive cells and autophagosome formation. The expression of Beclin-1 and the LC3-II/LC3-I ratio also increased as the starvation prolonged, reaching the peak levels at 3 h and 4 h, respectively. Conclusion Autophagy can be induced by starvation in A549 and 95D cells in correlation with the expression of autophagy-related proteins LC3 and Beclin-1. These cell models of nutritional deficiency-induced autophagy may allow for a better understanding of the role of autophagy in the development of non-small cell lung cancer.
Keywords：non-small cell lung cancerstarvationautophagyLC3Beclin1
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 627-630 )